Candida Species by Richard Calderone & Ronald Cihlar

Author:Richard Calderone & Ronald Cihlar
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

6.Keeping the sample on the magnet, air-dry the beads at room temperature for 3–5 min.

7.Remove the plate or tubes from the magnet, and add 40 μL low TE directly to the pellet to disperse the beads. Pipet the mixture up and down five times, then vortex the sample for 10 s, to mix thoroughly.

8.Place the plate or tubes on a magnet for at least 1 min. After the solution clears, transfer the supernatant containing the ligated/barcoded DNA library to a new plate.

Stopping Point: (Optional) Store the DNA at −10 to −30 °C. 9.Proceed to size-select the ligated/barcoded library, as described below.

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